Monopolar attachment of sister kinetochores at meiosis I requires casein kinase 1.

ABSTRACT: The segregation of maternal centromeres away from the paternal ones during the first division of meiosis depends on the attachment of sister kinetochores to microtubules emanating from the same spindle pole. In budding yeast monopolar attachment requires the recruitment to kinetochores of a protein complex called monopolin. The biochemical function of monopolin was unknown. Here, we have identified the casein kinase I Hrr25 as a hitherto unknown subunit of monopolin. Hrr25 differs from other monopolin components by its enzymatic activity and strong evolutionary conservation. We demonstrate that Hrr25’s kinase activity and its interaction with monopolin are both required for monopolar attachment. Accordingly, Hrr25 is associated with centromeres in meiosis I. Our results revealed a surpri

ORGANISM(S): Saccharomyces cerevisiae

SUBMITTER: Kim Nasmyth 

PROVIDER: E-GEOD-4792 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress