Global regulation of alternative splicing by adenosine deaminase acting on RNA (ADAR) [expression]

ABSTRACT: Alternative mRNA splicing is a major mechanism for gene regulation and transcriptome diversity. Despite the extent of the phenomenon, the regulation and specificity of the splicing machinery are only partially understood. Adenosine-to-inosine (A-to-I) RNA editing of pre-mRNA by ADAR enzymes has been linked to splicing regulation in several cases. Here we used bioinformatics approaches, RNA-seq and exon-specific microarray of ADAR knockdown cells to globally examine how ADAR and its A-to-I RNA editing activity influence alternative mRNA splicing. Although A-to-I RNA editing only rarely targets canonical splicing acceptor, donor, and branch sites, it was found to affect splicing regulatory elements (SREs) within exons. Cassette exons were found to be significantly enriched with A-to-I RNA ed

ORGANISM(S): Homo sapiens

SUBMITTER: jasmine jacob 

PROVIDER: E-GEOD-47945 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress