In vivo determination of candidate direct targets of the nonsense mediated decay pathway in intact Drosophila

ABSTRACT: Regulation of mRNA stability is a critical step in the control of gene expression. The nonsense mediated mRNA decay (NMD) pathway is one important mRNA stability pathway and has been shown to function in regulating a significant portion of the transcriptome. Perhaps the most significant outstanding question surrounding NMD regulation of endogenous gene expression is the identification of transcripts directly targeted by the NMD pathway versus secondary response genes, whose expression levels are dependent on the activity of direct targets. Here, we present for the first time in an intact animal, the experimental identification of direct targets of NMD. We find that most genes (74%) that are upregulated in NMD mutants appear not to be direct targets of NMD. More surprisingly, we find that t

ORGANISM(S): Drosophila melanogaster

SUBMITTER: Hao Hu 

PROVIDER: E-GEOD-47979 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress