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Metabolomics,Unknown,Transcriptomics,Genomics,Proteomics

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Transcription profiling of mouse fundic mucosa in the presence/absence of gastrin and in gastrin-CCK double knockout mice

ABSTRACT: Fundic mucosa gene expression in wildtype vs gastrin knockout, gastrin replaced gastrin KO and gastrin-CCK double knockout mice; Fundic mucosal scraping RNA were isolated from WT, Gastrin Knockout, Gastrin replaced KO and Gastrin-CCK double knockout mice. Targets from three biological replicates of each were generated and the expression profiles were determined using Affymetrix murine 430A arrays. Comparisons between the sample groups allow the identification of genes with gastrin responsiveness and Gastrin-CCK double effect . Experiment Overall Design: 3 WT, 3 Gastrin KO, 3 Gastrin replaced KO and 3 Gastrin-CCK double Knockout fundic RNA replicates were analyzed

ORGANISM(S): Mus musculus

SUBMITTER: Linda Samuelson

PROVIDER: E-GEOD-4810 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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Transcriptional profiling of gastrin-regulated genes in mouse stomach.

Jain Renu N RN Samuelson Linda C LC

Physiological genomics 20061114 1

Gastrin, a potent stimulator of gastric acid secretion, primarily targets the acid-secreting parietal cells and histamine-secreting enterochromaffin-like (ECL) cells in the stomach. Accordingly, gastrin-deficient (GAS-KO) mice have a severe impairment in acid secretion. The aim of this study was to characterize changes in gene expression in GAS-KO mice to identify gastrin-regulated genes and to gain insight into how gastric cell types are regulated by gastrin and acid secretion. Affymetrix micro ...[more]

PMID: 17105752

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Transcription profiling of parietal cells isolated from WT and Gastrin Knockout mice to identify genes responsive to gastrin.

Project description:Parietal cells were isolated from WT and Gastrin Knockout mice. Targets from three biological replicates of each were generated and the expression profiles were determined using Affymetrix murine U74Av2 arrays. Comparisons between the sample groups allow the identification of genes with gastrin responsiveness. Experiment Overall Design: 3 WT parietal cell and 3 Gastrin Kncokout parietal cell replicates were analyzed

2007-08-31 | E-GEOD-2772 | biostudies-arrayexpress

Fundic mucosa gene expression in the presence/absence of gastrin and in gastrin-CCK double knockout mice.

Project description:Fundic mucosa gene expression in wildtype vs gastrin knockout, gastrin replaced gastrin KO and gastrin-CCK double knockout mice Fundic mucosal scraping RNA were isolated from WT, Gastrin Knockout, Gastrin replaced KO and Gastrin-CCK double knockout mice. Targets from three biological replicates of each were generated and the expression profiles were determined using Affymetrix murine 430A arrays. Comparisons between the sample groups allow the identification of genes with gastrin responsiveness and Gastrin-CCK double effect . Keywords: repeat

2007-05-03 | GSE4810 | GEO

Transcription profiling of rat AR42J cells treated with gastrin at multiple time points

Project description:The gastric peptide hormone gastrin is an example of a stimulus that can mediate gene expression associated with different cell specific outcomes including apoptosis, proliferation, migration and differentiation. We perform transcriptional profiling of AR42J cells treated with gastrin in sustained mode, and harvested at 11 different time points between 0 and 24 hours. Cells treated with gastrin in transient mode (gastrin containing medium was removed after 2 hours and replaced with medium without gastrin) were harvested at 6 different time points between 4 and 24 h. The samples from unstimulated control cells were harvested at T0 and throughout the time course.

2010-01-01 | E-MTAB-123 | biostudies-arrayexpress

Parietal cell gene expression in the presence and absence of gastrin

2005-11-15 | GSE2772 | GEO

Exploring signal-induced cellular regulatory subnetworks by use of partial least square regression (PLSR) multidimensional analysis of gene expression time series data

Project description:By use of time series microarray experiment we identified the characteristic temporal behaviour of ~2000 new gastrin-regulated genes (accession numbers: GSE32869). To inform gastrin regulatory networks with an additional experimental dimension, we determined the temporal transcriptome in the presence of the protein synthesis inhibitor cycloheximide (CHX). For those genes where the gastrin response in the presence of CHX was higher than the response with CHX alone the gene was classified as a primary gene not dependent on de novo protein synthesis for gastrin-induced expression.Gastrin induced genes that were not upregulated in the presence of CHX were classified as secondary genes, i.e., genes whose transcriptional activation dependends on de novo protein synthesis of one or several factors that must be induced by gastrin previous to these secondary genes.

2012-10-01 | E-MTAB-1268 | biostudies-arrayexpress

Effects of a gastrin receptor antagonist (NTZ, previously named YF) on the gastric corpus mucosa of HKATPase betasubunit knockout (KO) mice

Project description:Purpose: the effects of a gastrin receptor antagonist has been studies in patients with gastrin neuroendocrine (NE) tumours due to hypergastrinemia. This mouse model of hypergastrinemia is a model for studying long term acid inhibition, including NE hyperplasia and formation of Spasmolytic polypeptide expressing metaplasia (SPEM), a proposed premalignant alteration of the stomach. Methods: RNA was isolated from the oxyntic mucosa. RNA was sequenced using standard Illumina protocols on a NextSeq 500 instrument. Results: NTZ reduced oxyntic mucosal hypertropohy and stomach weight. Neuroendocrine cell hyperplasia and NE markers were also reduced. Spasmolytic polypeptide expressing metaplasia (SPEM) was expressed in KO mice and was not affected by the gastrin receptor antagonist. Conclusions: The gastrin receptor antagonist YF reduced gastric corpus mucosal hypertrophy and NE cell hyperplasia in HKATPase KO mice, but did not affect SPEM or SPEM related genes.

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Gastrin, PAI-1 and mucosal growth. Whole organism studies in transgenic mice.

Project description:We are studying the role of PAI-1 in hypergastrinemic transgenic mice (PAI-1 knockout, HK-ATPase beta knockout and corresponding control strains). As a model of hypergastrinaemia, HK-ATPase beta knockout mice are preferred instead of giving a high dose of proton pump inhibitors daily. We have successfully crossed these two strains and generated double knockout mice (PAI-1--/HK-ATPase--) and 3 different control groups (PAI-1++/HK-ATPase++, PAI-1--/HK-ATPase++ and PAI-1++/HK-ATPase--) (9 animals in each group). The gastric phenotype of all mice has been examined by morphologic and molecular analysis after 12 months. Gastric biopsies and blood samples have been taken for histology, large scale gene expression analysis, verification of mRNA and protein expression and gastrin measurements. Global gene expression analysis has been done on gastric mucosal samples from all groups of mice. The differences between wild type mice and the transgenic animals are expected to provide novel hypotheses on the role of PAI-1 in gastrin-induced changes in the gastric mucosa.

2016-02-01 | E-MTAB-3853 | biostudies-arrayexpress

Mus musculus

Project description:Fundic mucosa gene expression in the presence/absence of gastrin and in gastrin-CCK double knockout mice.

| PRJNA95611 | ENA

Genome-wide time series analysis of gastrin induced gene expression in AR42J cells with and without protein synthesis

Project description:Genome-wide gene expression time series experiments are an important approach to studying a cells response to a given stimulus, as they enable capturing of the dynamics of the response. Moreover, information about the dynamics of the system is an important prerequisite for inferences on interactions between its components. We have performed extensive microarray-based genome-wide gene expression analyses up to 14h of the response to gastrin in AR42J adenocarcinoma cells. The gastrin reponse was also investigated in presence of the protein translation inhibitor cycloheximide. The rat cell line AR42J derived from pancreatic duct has the feature of pluripotency of the precursor cells of the gut endoderm. Furthermore, AR42J cells express gastrin receptors endogenously and are therefore frequently used as a model system to study gastrin responses. The hormone gastrin is the central regulator of gastric acid secretion and in addition plays a prominent role in regulation of growth and differentiation of gastric and colonic mucosa. Hypergastrinaemia has been linked to development of neuroendocrine gastrointestinal tumours (NE-GI) and, in conjugation with additional factors such as inflammation, to development of gastric adenocarcinoma (GA)

2011-10-17 | GSE32869 | GEO

Genome-wide time series analysis of gastrin induced gene expression in AR42J cells with and without protein synthesis

2011-10-16 | E-GEOD-32869 | biostudies-arrayexpress

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