Genome-wide mapping of CarD, RNAPs, and RNAPb on the Mycobacterium smegmatis genome using Chromatin Immunoprecipitation Sequencing

ABSTRACT: CarD is an essential mycobacterial protein that we had previously shown to bind the RNA polymerase (RNAP) and affect the transcriptional profile of M. smegmatis and Mycobacterium tuberculosis. For this reason, we suspected that CarD was directly regulating transcriptional complexes but we did not know at what stage of CarD was functioning and at which genes CarD interacted with the RNAP. To determine in which stage of the transcription cycle (initiation, elongation, or termination) CarD acts, we used Chromatin Immunoprecipitation sequencing (ChIP-seq) to survey the distribution of CarD throughout the M. smegmatis chromosome. Specific antibodies targeting core RNAPb, RNAPσ, or a hemagglutinin (HA) epitope fused to CarD (CarD-HA) were used to co-immunoprecipitate associated DNA. CarD-HA was

ORGANISM(S): Mycobacterium smegmatis

SUBMITTER: Bic MSKCC 

PROVIDER: E-GEOD-48164 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress