The dynamics of DNA methylation fidelity during mouse embryonic stem cell renewal and differentiation

ABSTRACT: Purpose: we aimed to gain a genome-wide view of the dynamics in DNA methylation inheritance and define the factors associated with methylation fidelity. Methods: Using mouse embryonic stem cell (ES-E14TG2a) in both undifferentiated and differentiated states as a model system, we exploited the hairpin bisulfite sequencing approach to generate methylation data for DNA double strands simultaneously at single-base resolution. We generated the genome-wide hairpin bisulfite sequencing data to capture the methylation pattern variation during the stem cell transition from self-renewal to commitment, and integrated with various M-bM-^@M-^\omicsM-bM-^@M-^] data to scrutinize the relationships among DNA methylation inheritance, gene expression, histone modification, transcriptional factor binding and

ORGANISM(S): Mus musculus

SUBMITTER: Mingan Sun 

PROVIDER: E-GEOD-48229 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress