Analysis of a fungus-specific transcription factor family, the Candida albicans zinc cluster proteins, by artificial activation

ABSTRACT: The zinc cluster proteins are a family of transcription factors that are unique to the fungal kingdom. In the pathogenic yeast Candida albicans, zinc cluster transcription factors control the expression of virulence-associated traits and play key roles in the development of antifungal drug resistance. Gain-of-function mutations in several zinc cluster transcription factors, which result in constitutive overexpression of their target genes, are a frequent cause of azole resistance in clinical C. albicans isolates. We found that zinc cluster proteins can also be artificially activated by C-terminal fusion with the heterologous Gal4 activation domain. We used this strategy to create a comprehensive library of C. albicans strains expressing all 82 zinc cluster transcription factors of this fun

ORGANISM(S): Candida albicans

SUBMITTER: Rebecca Schillig 

PROVIDER: E-GEOD-48259 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress