Single cell Hi-C reveals cell-to-cell variability in chromosome structure

ABSTRACT: Large-scale chromosome structure and spatial nuclear arrangement have been linked to control of gene expression and DNA replication and repair. Genomic techniques based on chromosome conformation capture assess contacts for millions of loci simultaneously, but do so by averaging chromosome conformations from millions of nuclei. Here we introduce single cell Hi-C, combined with genome-wide statistical analysis and structural modeling of single copy X chromosomes, to show that individual chromosomes maintain domain organisation at the megabase scale, but show variable cell-to-cell chromosome territory structures at larger scales. Despite this structural stochasticity, localisation of active gene domains to boundaries of territories is a hallmark of chromosomal conformation, affecting most do

ORGANISM(S): Mus musculus

SUBMITTER: Yaniv Lubling 

PROVIDER: E-GEOD-48262 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress