Metabolomics,Unknown,Transcriptomics,Genomics,Proteomics

Dataset Information

0

Expression data from human sarcoma patient samples treated with either vehicle control or Nutlin-3a

ABSTRACT: This study has examined the molecular mechanisms underlying sensitivity of sarcomas to Nutlin-3a, a non-genotoxic activator of the p53 pathway. Human patient material was collected immediately following surgical resection, dissected into small pieces and ex planted onto gelatin sponges immersed in media containing either vehicle control or Nutlin-3a (10uM and/or 50uM) for 48 hours. Nutlin-3a represents a novel targeted therapy for the treatment of sarcomas. We have examined expression profiles of genes upregulated in sarcoma patient derived tissues following nutlin-3a treatment ex vivo 16 samples ((Vehicle control and Nutlin-3a (10uM and 50uM) treated samples)) from 6 sarcoma patients

ORGANISM(S): Homo sapiens

SUBMITTER: Kathleen Pishas 

PROVIDER: E-GEOD-48296 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

Json Xml
altmetric image

Publications

Nutlin-3a is a small-molecule antagonist of p53/MDM2 that is being explored as a treatment for sarcoma. In this study, we examined the molecular mechanisms underlying the sensitivity of sarcomas to Nutlin-3a. In an ex vivo tissue explant system, we found that TP53 pathway alterations (TP53 status, MDM2/MDM4 genomic amplification/mRNA overexpression, MDM2 SNP309, and TP53 SNP72) did not confer apoptotic or cytostatic responses in sarcoma tissue biopsies (n = 24). Unexpectedly, MDM2 status did not  ...[more]

Similar Datasets

Unknown Expression data from human sarcoma patient samples treated with either vehicle control or Nutlin-3a
2014-03-10 | GSE48296 | GEO
Transcriptomics Transcriptome-profiling (RNA-seq) and Ribosome-profiling (Ribo-seq) of BJ cells treated with Nutlin-3a, an MDM2 inhibitor, which induces p53.
2013-06-01 | E-GEOD-45785 | biostudies-arrayexpress
Unknown Transcriptomics analysis in 3T3L1 preadipocytes with activation of p53 via Nutlin-3a treatment
2018-07-05 | GSE100811 | GEO
Proteomics Nutlin-3a-induced changes in the proteome of parental and RFX7 knock-out U2OS cells
2023-03-11 | PXD024869 | Pride
Transcriptomics MicroRNA profiling by array of human multiple myloma p53 wild type cells treated with nutlin 3A
2010-07-22 | E-TABM-1038 | biostudies-arrayexpress
Unknown mRNA-seq from Nutlin-3a, doxorubicin, and DMSO treated HCT116 p21-/- cells
2016-06-09 | GSE77841 | GEO
Unknown Nutlin-3a induced changes in the transcriptome of parental and RFX7-/- U2OS cells
2023-02-28 | GSE173483 | GEO
Unknown Cooperation of Nutlin-3a and a Wip1 inhibitor to induce p53 activity
2016-05-02 | GSE80716 | GEO
Unknown PolyA RNA-seq from CHP212 WT and FAM193AKO cells treated with Nutlin-3
2022-12-19 | GSE189133 | GEO
Proteomics Interruption of p53-MDM2 Interaction by Nutlin-3a in Human Lymphoma Cell Models Initiates a Cell-Dependent Global Effect on Transcriptome and Proteome Level
2023-07-30 | PXD009290 | Pride