High-Resolution Profiling of Novel Transcribed Regions During Rat Spermatogenesis

ABSTRACT: Mammalian spermatogenesis is a complex and highly orchestrated combination of processes in which male germline proliferation and differentiation result in the production of mature spermatozoa. If recent genome-wide studies have contributed to the in-depth analysis of the male germline protein-coding transcriptome, little effort has yet been devoted to the systematic identification of novel unannotated transcribed regions expressed during mammalian spermatogenesis. We report high-resolution expression profiling of male germ cells in the rat using Illumina next-generation sequencing technology and highly enriched testicular cell populations. Among 20,424 high-confidence transcripts reconstructed, we defined a stringent set of 1,419 long multi-exonic unannotated transcripts expressed in the t

ORGANISM(S): Rattus norvegicus

SUBMITTER: Frédéric Chalmel 

PROVIDER: E-GEOD-48321 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress