S-adenosylmethionine modifies cocaine-induced DNA methylation and increases locomotor sensitization in mice

ABSTRACT: The aim of the study was to investigate whether environmental factors like S-adenosylmethionine (SAM) via affecting epigenome could alter cocaine-induced gene expression and locomotor sensitization in mice. Using mouse nucleus accumbens (NAc) tissue, whole-genome gene expression profiling revealed that repeated SAM treatment affected a limited number of genes, but significantly modified cocaine-induced gene expression by blunting nonspecifically the cocaine response. At the gene level, we discovered that SAM modulated cocaine-induced DNA methylation by inhibiting both promoter-associated CpG-island hyper- and hypomethylation in the NAc but not in the reference tissue cerebellum. Total RNA was extracted from the mouse nucleus accumbens (NAc) tissue. Two tissues were combined to a sample, 4

ORGANISM(S): Mus musculus

SUBMITTER: Kaili Anier 

PROVIDER: E-GEOD-48365 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress