Metabolomics,Unknown,Transcriptomics,Genomics,Proteomics

Dataset Information

Molecular Insight into the Association between Cartilage Regeneration and Ear Wound Healing in Genetic Mouse Models: Targeting New Genes in Regeneration

ABSTRACT: Certain mouse strains have the ability to heal both ear wounds and knee articular cartilage injuries. A positive genetic correlation between cartilage healing and protection from osteoarthritis exists, which suggests that a common core of genes may operate in healing process. Here, we analyzed knee joint tissues from healer and non-healer mice and found that four genes were common to both ear wound and knee cartilage healing. Specific sequence differences between haler and non-healer parental strains were identified as potential causal polymorphisms. We suggest a common molecular and genetic basis of tissue healing. We collected tissue lysates from the formalin-fixed paraffin-embedded sections from mouse knee joints and analyzed the expression of several genes by Affymetrix QunatiGene Plex assay. Please note that 8-9 replicates were performed per each sample. The averaged (across the replicate samples) data was provided for each sample record and non-averaged data (from each replicate) is provided as a Series supplementary file (*setI.txt contains data from Panel 321347 and *setII.txt from panel 321389).

ORGANISM(S): Mus musculus

SUBMITTER: Muhammad Farooq Rai

PROVIDER: E-GEOD-48505 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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Publications

Molecular insight into the association between cartilage regeneration and ear wound healing in genetic mouse models: targeting new genes in regeneration.

Rai Muhammad Farooq MF Schmidt Eric J EJ McAlinden Audrey A Cheverud James M JM Sandell Linda J LJ

G3 (Bethesda, Md.) 20131106 11

Tissue regeneration is a complex trait with few genetic models available. Mouse strains LG/J and MRL are exceptional healers. Using recombinant inbred strains from a large (LG/J, healer) and small (SM/J, nonhealer) intercross, we have previously shown a positive genetic correlation between ear wound healing, knee cartilage regeneration, and protection from osteoarthritis. We hypothesize that a common set of genes operates in tissue healing and articular cartilage regeneration. Taking advantage o ...[more]

PMID: 24002865

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Dataset Information

Molecular Insight into the Association between Cartilage Regeneration and Ear Wound Healing in Genetic Mouse Models: Targeting New Genes in Regeneration

Publications

Molecular insight into the association between cartilage regeneration and ear wound healing in genetic mouse models: targeting new genes in regeneration.

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