Genome-wide methylation maps for Proliferating and Senescent cells

ABSTRACT: Altered DNA methylation and associated destabilization of genome integrity and function is a hallmark of cancer. Replicative senescence imposes a limit on proliferative potential that all cancer cells must bypass. Compared to proliferating cells, senescent cells exhibit marked chromatin re-organization. Here we show by whole-genome single-nucleotide bisulfite sequencing that replicative senescent human cells exhibit widespread alterations in their DNA methylome. These changes are linked to mislocalization of the maintenance DNA methyltransferase (DNMT1) in cells approaching senescence, altered replication-coupled DNA methylation and de-repression of repetitive satellite sequences. Deficiency of DNMT1 triggers chromatin changes characteristic of senescence and expression of satellite sequen

ORGANISM(S): Homo sapiens

SUBMITTER: Tony McBryan 

PROVIDER: E-GEOD-48580 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress