Effects of 5M-NM-1-dihydrotestosterone on male mummichog (Fundulus heteroclitus) testes steroid production and gene expression patterns

ABSTRACT: To better assess the impact of contaminants on fish reproduction, it is increasingly important to collect data at multiple levels of biological organization. However, a challenge in ecotoxicology is that three of the most commonly measured reproductive endpoints; gonad histology, ex vivo steroid production, and gene expression, can be difficult to obtain in small-bodied fish (e.g. zebrafish, Japanese medaka, fathead minnow) due to limited tissue availability. Using 5M-NM-1-dihydrotestosterone (DHT) as a test compound, the objectives of the current study were to assess the feasibility of measuring gene expression in tissues also used for ex vivo steroid production studies. A portion of the testes was also used for qualitative histological examination. Male mummichog were exposed to 0, 5,

ORGANISM(S): Fundulus heteroclitus

SUBMITTER: Christopher Martyniuk 

PROVIDER: E-GEOD-48582 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress