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Metabolomics,Unknown,Transcriptomics,Genomics,Proteomics

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Genome-wide analysis of keratinocyte gene expression during their course of differentiation in presence or absence of JNK1,2-deficient or wildtype immortalized mouse embryonic fibroblasts (MEFs)

ABSTRACT: Analysis of keratinocyte differentiation in presence or absence of fibroblast-derived JNK-dependent soluble factors in vitro. The hypothesis tested in the present study was that JNK-dependent fibroblast-derived soluble factors promote an efficient keratinocyte differentiation. Results provide important information about reduced expression of a subset of differentiation associated genes in keratinocytes in the absence of JNK-dependent fibroblast-derived soluble factors. In vitro transwell co-culture experiments were performed using jnk1-/-jnk2-/- or wildtype immortalized mouse embryonic fibroblasts (MEFs) and differentiating primary normal human epidermal keratinocytes (NHEK) over a time course of 6 days. Total RNA was obtained from NHEK prior to cultivation with MEFs (day 0) and every second day (days 2, 4, and 6) during co-culture. MEFs have been described previously in Sabapathy et al. (2004) Mol Cell 15:713-25.

ORGANISM(S): Homo sapiens

SUBMITTER: Peter Angel

PROVIDER: E-GEOD-48613 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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Genome-wide analysis of gene expression in jnk1-/-jnk2-/- immortalized mouse embryonic fibroblasts compared to wildtype counterparts co-cultivated with differentiating keratinocytes

Project description:Analysis of JNK-dependent fibroblast-derived soluble factors at gene expression level. The hypothesis tested in the present study was that loss of c-Jun N-terminal kinases 1 and 2, JNK1 and JNK2, in MEFs causes a strong alteration of the gene expression program coding for soluble factors, which promote an efficient keratinocyte differentiation. Results provide important information of the repertoire of fibroblast transcripts encoding secreted proteins, which is severely disarranged upon loss of JNK activity under the in vitro co-culture conditions applied. In vitro transwell co-culture experiments were performed using jnk1-/-jnk2-/- or wildtype immortalized mouse embryonic fibroblasts (MEFs) and differentiating primary normal human epidermal keratinocytes (NHEK) over a time course of 6 days. Every second day, fibroblast-loaded inserts were changed resulting in 3 triads (triads 1, 2, and 3). Total RNA was obtained from jnk1-/-jnk2-/- and wildtype immortalized mouse embryonic fibroblasts (MEFs) prior to co-cultivation (day 0) and of each triad 1, 2, or 3.

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Project description:This SuperSeries is composed of the SubSeries listed below. Refer to individual Series

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Genome-wide analysis of keratinocyte gene expression during their course of differentiation in presence or absence of JNK1,2-deficient or wildtype immortalized mouse embryonic fibroblasts (MEFs)

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Genome-wide analysis of gene expression in jnk1-/-jnk2-/- immortalized mouse embryonic fibroblasts compared to wildtype counterparts co-cultivated with differentiating keratinocytes

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