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Transcription profiling of human galactose-1-phosphate uridyltransferase (GALT)-deficient cells reveals novel mechanisms of galactose toxicity

ABSTRACT: Comparison of gene expression profiles in GALT-deficient and GALT-reconstituted cells showed that cells lacking GALT activity responded to galactose challenge by activating a set of genes characteristic of endoplasmic reticulum (ER) stress. This response was specific to galactose insult, as cells grown in glucose or hexose-free media did not exhibit ER stress. Experiment Overall Design: Cells were first grown and then infected in high glucose, then transferred to DMEM, 3 experiments with dye-swap

ORGANISM(S): Homo sapiens

SUBMITTER: Kent Lai

PROVIDER: E-GEOD-4864 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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Novel mechanisms of galactose toxicity in human galactose-1-phosphate uridyltransferase (GALT)-deficient cells

Project description:Comparison of gene expression profiles in GALT-deficient and GALT-reconstituted cells showed that cells lacking GALT activity responded to galactose challenge by activating a set of genes characteristic of endoplasmic reticulum (ER) stress. This response was specific to galactose insult, as cells grown in glucose or hexose-free media did not exhibit ER stress. Keywords: GALT infected cells

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