An improved SELEX-Seq strategy for characterizing DNA-binding specificity of transcription factor: NF-M-NM-:B as an example

ABSTRACT: We provided an improved SELEX-Seq strategy for characterizing DNA-binding specificity of transcription factor. We valided the strategy by characterzing the DNA-binding specificty of NF-M-NM-:B p50 dimer. Proteins of the Nf-kappab family were bound to DNA oligonucleotides containing a degenerate region. The protein-DNA complexes were selected after one or multiple rounds of SELEX and the DNA molecules were deep sequenced.

ORGANISM(S): Homo sapiens

SUBMITTER: Jinke Wang 

PROVIDER: E-GEOD-48660 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress