Global response to chemotherapy-induced apoptosis

ABSTRACT: Here we use an integrated systems-level examination of transcription, translation, and proteolysis to explore how cancer cells struggle with a chemotherapeutic drug prior to succumbing to apoptosis. As a model system we study myeloma cells exposed to the proteasome inhibitor bortezomib, a first-line clinical treatment. Despite robust transcriptional changes, unbiased quantitative proteomics detects production of only a few critical anti-apoptotic proteins against a background of general translation inhibition. Ribosome profiling further reveals potential translational regulation of stress response genes following bortezomib treatment. Once the apoptotic machinery is engaged, degradation by caspases is largely independent of changes at the transcriptional level. Moreover, previously unchara

ORGANISM(S): Homo sapiens

SUBMITTER: Arun Wiita 

PROVIDER: E-GEOD-48785 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress