RNA-seq reveals differential gene expression in Staphylococcus aureus at single-nucleotide resolution

ABSTRACT: Staphylococcus aureus is a gram-positive cocci and an important human commensal bacteria and pathogen. S. aureus infections are increasingly difficult to treat because of the emergence of highly resistant MRSA (Methicillin-resistant S. aureus) strains. Here we present a method to study differential gene expression in S. aureus using high-throughput RNA-sequencing (RNA-seq). We use RNA-seq to examine the differential gene expression in S. aureus RN4220 cells containing an exogenously expressed transcription factor and between two S. aureus strains (RN4220 and NCTC8325-4). The information provided by RNA-seq was a significant advance over previously described microarray based techniques. We investigated the sequence and gene expression differences between RN4220 and NCTC8325-4 and used th

ORGANISM(S): Staphylococcus aureus

SUBMITTER: Joseph Osmundson 

PROVIDER: E-GEOD-48896 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress