Comparison of RNA-Seq and Microarray in Transcriptome Profiling During T Cell Activation

ABSTRACT: Samples in this study probe the gene expression kinetics in human CCR6+ Th17 memory T cells activated under Th17 condition. Human CCR6+ Th17 memory T cells were purified from PBMC and gene expression was studied over a time course of 3 days after activation under Th17 condition. RNA from these samples was also profiled using RNA-Seq to compare different transcriptome profiling technologies. PBMCs were purified from whole blood from a healthy donor. CD4+ cells were isolated by negative selection, followed by CCR6+ purification by positive selection. Two replicates of 6 time points were taken following stimulation in Th17 polarizing conditions. This submission represents microarray component of study.

ORGANISM(S): Homo sapiens

SUBMITTER: Anton Bittner 

PROVIDER: E-GEOD-48978 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress