RNA-sequencing of Nedd4+/+ and Nedd4-/- mouse splenic B cells

ABSTRACT: Programmed mutagenesis of the immunoglobulin locus of B-lymphocytes during class switch recombination and somatic hypermutation requires RNA polymerase II (RNA polII) transcription complex dependent targeting of the DNA mutator, Activation Induced cytidine Deaminase (AID). AID deaminates cytidine residues on substrate sequences in the immunoglobulin (Ig) locus via a transcription-dependent mechanism and this activity is  stimulated by the RNA polII stalling co-factor Spt5 and the eleven-subunit cellular non-coding RNA 3’-5’ exonucleolytic processing complex, RNA exosome. The mechanism by which the RNA exosome recognizes immunoglobulin locus RNA substrates to stimulate AID DNA deamination activity on its in vivo substrate sequences is an important question. Here we report that E3-ubiquitin

ORGANISM(S): Mus musculus

SUBMITTER: Jiguang Wang 

PROVIDER: E-GEOD-49027 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress