Aging-induced differential methylation in human PBMCs occurs with but often without change in expression of the associated gene (Expression)

ABSTRACT: Aging is a progressive process that results in the accumulation of intra- and extracellular alterations that in turn contribute to a reduction in health. Age-related changes in DNA methylation have been reported before and may be responsible for aging-induced changes in gene expression, although a causal relationship has yet to be shown. Using genome-wide assays, we analyzed age-induced changes in DNA methylation and their effect on gene expression with and without transient induction with the synthetic transcription modulating agent WY14,643. To demonstrate feasibility of the approach, we isolated peripheral blood mononucleated cells (PBMCs) from five young and five old healthy male volunteers and cultured them with or without WY14,643. Infinium 450K BeadChip and Affymetrix Human Gene 1.1

ORGANISM(S): Homo sapiens

SUBMITTER: Guido Hooiveld 

PROVIDER: E-GEOD-49058 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress