Comparative gene expression analyses using post-implanted E6.5 cloned embryos

ABSTRACT: The great majority of embryos generated by somatic cell nuclear transfer (SCNT) display defined abnormal phenotypes after implantation, such as an increased likelihood of death and abnormal placentation. To gain better insight into the underlying mechanisms, we analyzed genome-wide gene expression profiles of day 6.5 postimplantation mouse embryos cloned from three different cell types (cumulus cells, neonatal Sertoli cells and fibroblasts). The embryos retrieved from the uteri were separated into embryonic (epiblast) and extraembryonic (extraembryonic ectoderm and ectoplacental cone) tissues and were subjected to gene microarray analysis. Genotype- and sex-matched embryos produced by in vitro fertilization (IVF) were used as controls. Principal component analysis revealed that whereas the

ORGANISM(S): Mus musculus

SUBMITTER: Kimiko Inoue 

PROVIDER: E-GEOD-49171 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress