Ontogeny, distribution and potential roles of hydroxymethylcytosine in human liver function

ABSTRACT: Background: Interindividual differences in liver functions such as protein synthesis, lipid and carbohydrate metabolism and drug metabolism are influenced by epigenetic factors. The role of the epigenetic machinery in such processes has, however, been barely investigated. 5-hydroxymethylcytosine (5hmC) is a recently re-discovered epigenetic DNA modification that plays an important role in the control of gene expression. Results: In this study, we investigate 5hmC occurrence and genomic distribution in 8 fetal and 7 adult human liver samples in relation to ontogeny and function. LC-MS analysis shows that in the adult liver samples 5hmc comprises up to 1% of the total cytosine content, whereas in all fetal livers it is below 0.125%. Immunohistostaining of liver sections with a polyclonal ant

ORGANISM(S): Homo sapiens

SUBMITTER: Mart Kals 

PROVIDER: E-GEOD-49291 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress