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CTCL and ATL cell lines, mir-150 transfected versus controls

ABSTRACT: We examined the function of miR-150 in T-cell lymphomagenesis. We transfected GFP-control or GFP-miR-150 into several T-cell lymphoma lines and sought which genes were regulated by miR-150. To examine the function of miR-150 in T-cell lymphomagenesis, we first transfected GFP-control (Mock) or GFP-miR-150 into several T-cell lymphoma lines (ATN-1, HUT78, My-La, and HH). To detect genes commonly downregulated among these cells, we used the CodeLinkTM Human Whole Genome Bioarray platform with the criterion that the miR-150/GFP-control ratio must be <0.75 in all 3 CTCL lines.

ORGANISM(S): Homo sapiens

SUBMITTER: Sho Ikeda

PROVIDER: E-GEOD-49308 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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CTCL and ATL cell lines, mir-150 transfected versus controls

Project description:We examined the function of miR-150 in T-cell lymphomagenesis. We transfected GFP-control or GFP-miR-150 into several T-cell lymphoma lines and sought which genes were regulated by miR-150.

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Gene expression analysis to identify target genes of miR-150 in Hodgkin lymphoma cell lines

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Project description:MYC regulates the expression of multiple microRNA (miRNA) genes and defines the Burkitt lymphoma (BL) miRNA signature. Here, we investigate the role of the MYC-regulated miRNAs by gain- and loss-of-function analysis. Overexpression of 5 miRNAs that were significantly downregulated by MYC resulted in strong (miR-150, miR-26a, miR-26b) and mild (miR-29a, let-7a) impaired cell growth. Overexpression of miR-155 increased proliferation of BL cells. By RNA immunoprecipitation of Argonaute 2 in BL cells with and without miR-155 we identified 54 miR-155 target genes. Using an shRNA approach we identified TBRG1 (NIAM1) as a miR-155 target gene that copied the miR-155-induced phenotype upon its inhibition. Analysis of TBRG1 protein expression and miR-155 levels in primary cases of B-cell lymphoma revealed that miR-155 levels are significantly lower in TBRG1 positive cases suggesting that TBRG1 is also regulated by miR-155 in primary B-cell lymphoma. Our data demonstrate that overexpression of individual MYC-repressed miRNAs has a strong suppressive effect on BL cell growth, whereas overexpression of miR-155 enhances B-cell lymphoma growth by targeting the tumor suppressor gene TBRG1. Gene expression profile was performed in ST486 Burkitt lymphoma cell line in 4 samples: ST486 EV (empty MXW-PGK-IRES-GFP vector) total cell lysate, ST486 EV Ago2-IP, ST486 miR-155 (ST486 with ectopic miR-155) total cell lysate, ST486 miR-155 Ago2-IP.

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Gene expression of CTCL cell line MyLa transduced with Empty-GFP or miR-26a-GFP expressing vector

Project description:By using a CTCL cell line MyLa transduced Empty-GFP or miR-26a-GFP, we conducted whole genome gene screening against the cells.

2021-07-21 | GSE180472 | GEO

Diagnostic microRNA profiling in cutaneous T-cell lymphoma (CTCL)

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Gene expression analysis to identify target genes of miR-150 in Hodgkin lymphoma cell lines

Project description:Gene expression analysis to identify target genes of miR-150 in Hodgkin lymphoma cell lines

| PRJNA778243 | ENA

Restoring miR-150-5p to a triple negative breast cancer cell line [BT-549]

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