Gene expression of T follicular helper (TFH) cells 6 h after ICOS-L blockade

ABSTRACT: The maintenance of the TFH phenotype depends on continuous signals via ICOS. For a global assessment of differences in gene expression after interruption of the ICOS pathway a genome wide transcriptome analysis was performed. We used the OT-II adoptive transfer system to isolate antigen-specific TFH cells (day 6 after immunization) after short-term (6 hours) blockade of the ICOS pathway using a monoclonal antibody against ICOS-L. Gene expression profiles of TFH cells with or without ICOS-L blockade. Affymetrix MG 430 2.0 whole genome arrays were performed in duplicates for the control and blockade group (4 arrays in total). To obtain genes significantly regulated upon ICOS L blockade, the expression profiles of TFH cells treated with an isotype control or anti-ICOS-L antibody were compared

ORGANISM(S): Mus musculus

SUBMITTER: Joachim GrM-CM-<n 

PROVIDER: E-GEOD-49314 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress