Transcription profiling of naive CD4+ and CD8+ T-cells from Gfi1-knockout and wild type mice

ABSTRACT: to compare the transcriptomes of naïve CD4+ and CD8+ T-cells from Gfi1-knockout cells with wildtype cells, spleenocytes from wildtype and Gfi-knockout C57Bl/6 mice were isolated, stained with CD44, CD4 and CD8 antibodies and separated by using a FACS Diva (Becton Dickinson). Total RNAs isolated from 2 mice each (approximately 2xE06 cells) were pooled and subjected to microarray analysis on Affymetrix MOE430A_2.0 arrays Experiment Overall Design: 4 samples, 1 replicate per group

ORGANISM(S): Mus musculus

SUBMITTER: Ludger Klein-Hitpass 

PROVIDER: E-GEOD-4938 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress