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Metabolomics,Unknown,Transcriptomics,Genomics,Proteomics

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Transcription profiling of naive CD4+ and CD8+ T-cells from Gfi1-knockout and wild type mice

ABSTRACT: to compare the transcriptomes of naÃ¯ve CD4+ and CD8+ T-cells from Gfi1-knockout cells with wildtype cells, spleenocytes from wildtype and Gfi-knockout C57Bl/6 mice were isolated, stained with CD44, CD4 and CD8 antibodies and separated by using a FACS Diva (Becton Dickinson). Total RNAs isolated from 2 mice each (approximately 2xE06 cells) were pooled and subjected to microarray analysis on Affymetrix MOE430A_2.0 arrays Experiment Overall Design: 4 samples, 1 replicate per group

ORGANISM(S): Mus musculus

SUBMITTER: Ludger Klein-Hitpass

PROVIDER: E-GEOD-4938 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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Differential impact of the transcriptional repressor Gfi1 on mature CD4+ and CD8+ T lymphocyte function.

Pargmann Denise D Yücel Raif R Kosan Christian C Saba Ingrid I Klein-Hitpass Ludger L Schimmer Simone S Heyd Florian F Dittmer Ulf U Möröy Tarik T

European journal of immunology 20071201 12

The transcriptional repressor Gfi1 is a nuclear zinc-finger protein that is expressed in T cell precursors in the thymus, but is down-regulated in mature, resting T cells. Gfi1 expression rises transiently to levels seen in thymocytes upon antigenic activation. We show here that lack of Gfi1 causes delayed cell cycle entry and apoptosis after antigenic stimulation in both mature CD4+ and CD8+ T cells ex vivo. DNA micro-array analysis demonstrated that this correlated with an up-regulation of the ...[more]

PMID: 18034420

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murine CD4+ and CD8+ T-cells from wildtype and Gfi knockout cells

Project description:to compare the transcriptomes of naïve CD4+ and CD8+ T-cells from Gfi1-knockout cells with wildtype cells, spleenocytes from wildtype and Gfi-knockout C57Bl/6 mice were isolated, stained with CD44, CD4 and CD8 antibodies and separated by using a FACS Diva (Becton Dickinson). Total RNAs isolated from 2 mice each (approximately 2xE06 cells) were pooled and subjected to microarray analysis on Affymetrix MOE430A_2.0 arrays Keywords: knockout mice

2008-07-22 | GSE4938 | GEO

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2009-08-07 | E-GEOD-4950 | biostudies-arrayexpress

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Project description:To compare the transcriptomes of T-cells from Gfi1-knockout cells with wildtype cells, spleenocytes from wildtype and Gfi-knockout C57Bl/6 mice were isolated using panT-cell kit in an AutoMACS device (Miltenyi). Cells were cultured with anti-CD3 plus anti-CD28 antibodies (2 Âµg antibody/ml) for the indicated time period. Total RNAs was isolated and subjected to microarray analysis on Affymetrix MOE430A_2.0 arrays. Experiment Overall Design: 8 samples, 1 replicate per group

2008-10-18 | E-GEOD-4940 | biostudies-arrayexpress

murine T-cells from wildtype and Gfi1 knockout mice activated by anti-CD3 plus anti-CD28 for 0 to 24h

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2008-07-22 | GSE4940 | GEO

GFI1 tethers the NuRD complex to open and transcriptionally active chromatin in myeloid progenitors

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Project description:murine CD4+ and CD8+ T-cells from wildtype and Gfi knockout cells

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Gfi1 regulates survival and lineage commitment of hematopoietic precursors and prevents myeloproliferative diseases

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GFI deficiency in plasmacytoid dendritic cells

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