Genome-wide maps of HMGD1 and H1-bound nucleosomes.

ABSTRACT: Chromatin architectural proteins interact with nucleosomes to modulate chromatin accessibility and higher-order chromatin structure. While these proteins are almost certainly important for gene regulation they have been studied far less than the core histone proteins. Here we describe the genomic distributions and functional roles of two chromatin architectural proteins: histone H1 and the high mobility group protein HMGD1, in Drosophila S2 cells. Using ChIP-seq, biochemical and gene specific approaches, we find that HMGD1 binds to highly accessible regulatory chromatin and active promoters. In contrast, H1 is primarily associated with heterochromatic regions marked with repressive histone marks. However, the ratio of HMGD1 to H1 is better correlated with chromatin accessibility, gene expr

ORGANISM(S): Drosophila melanogaster

SUBMITTER: Graham McVicker 

PROVIDER: E-GEOD-49526 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress