Characterization of the Imprinting Signature of Mouse Embryo Fibroblasts by RNA Deep Sequencing

ABSTRACT: Mouse embryo fibroblasts (MEFs) closely resemble mouse embryos and are convenient sources for biochemical studies when cell number may be limiting from mouse embryos. To derive the imprinting signature of MEFs and potentially detect novel imprinted genes we characterized them using strand- and allele-specific RNA deep sequencing. We used Sequenom allelotyping in embryo and adult organs to verify parental allele-specific expression patterns. We found correct parental allele-specific transcription of 32 known ubiquitously imprinted genes in MEFs. Our analysis did not reveal any novel imprinted genes in MEFs, but detected extended parental allele-specific transcription in several known imprinted domains: maternal allele-specific transcription downstream of Grb10 and downstream of Meg3, Rtl1as

ORGANISM(S): Mus musculus

SUBMITTER: Xiwei Wu 

PROVIDER: E-GEOD-49538 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress