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Metabolomics,Unknown,Transcriptomics,Genomics,Proteomics

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Stretch responsive miRNAs in human aortic valve interstitial cells

ABSTRACT: miRNA-Sequencing was performed on human aortic valve interestitial cells (AVICs) exposed to 14% stretch at 1 hz or static conditions for 24h. Six static control and six samples exposed to cyclic stretch 14% for 24h

ORGANISM(S): Homo sapiens

SUBMITTER: Vishal Nigam

PROVIDER: E-GEOD-49558 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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The stretch responsive microRNA miR-148a-3p is a novel repressor of IKBKB, NF-κB signaling, and inflammatory gene expression in human aortic valve cells.

Patel Vishal V Carrion Katrina K Hollands Andrew A Hinton Andrew A Gallegos Thomas T Dyo Jeffrey J Sasik Roman R Leire Emma E Hardiman Gary G Mohamed Salah A SA Nigam Sanjay S King Charles C CC Nizet Victor V Nigam Vishal V

FASEB journal : official publication of the Federation of American Societies for Experimental Biology 20150128 5

Bicuspid aortic valves calcify at a significantly higher rate than normal aortic valves, a process that involves increased inflammation. Because we have previously found that bicuspid aortic valve experience greater stretch, we investigated the potential connection between stretch and inflammation in human aortic valve interstitial cells (AVICs). Microarray, quantitative PCR (qPCR), and protein assays performed on AVICs exposed to cyclic stretch showed that stretch was sufficient to increase exp ...[more]

PMID: 25630970

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Stretch responsive miRNAs in human aortic valve interstitial cells

Project description:miRNA-Sequencing was performed on human aortic valve interestitial cells (AVICs) exposed to 14% stretch at 1 hz or static conditions for 24h.

2015-01-09 | GSE49558 | GEO

Comparison of human aortic valve interstitial cells (AVICs) exposed to cyclic stretch to static samples

Project description:AVICs were exposed to cyclic stretch to examine the role of mechanical stimuli on gene expression AVICs cultured on collagen 1 coated Bioflex were exposed to 14% stretch at 1 hz or static conditions using a Flexcell-5000 14% stretch was the experimental condition while the static condition was the control

2015-01-09 | E-GEOD-47687 | biostudies-arrayexpress

Comparison of human aortic valve interstitial cells (AVICs) exposed to cyclic stretch to static samples

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Identification of Stretch Responsive microRNAs in Embyonic Mouse Cardiomyocytes

Project description:The goal of this study was to identify microRNAs that are modulated by cyclic stretch. miRNA-Seq was performed comparing samples from cultured E16.5 mouse cardiomyocytes exposed to either cyclic stretch of 16% at 1Hz for 24h or static conditions.

2020-01-22 | GSE120676 | GEO

The long non-coding HOTAIR is modulated by cyclic stretch and WNT/beta-CATENIN in human aortic valve cells and is a novel repressor of calcification genes

Project description:Aortic valve calcification is a significant and serious clinical problem for which there are no effective medical treatments. Individuals born with bicuspid aortic valves, 1-2% of the population, are at the highest risk of developing aortic valve calcification. Aortic valve calcification involves increased levels of calcification and inflammatory genes. Bicuspid aortic valve leaflets experience increased strain. The molecular mechanisms involved in the pathogenesis of calcification of BAVs are not well understood, especially the molecular response to mechanical stretch. HOTAIR is a long non-coding RNA (lncRNA) that has been implicated with cancer but has not been studied in cardiac disease. We have found that HOTAIR levels are decreased in BAVs and in human aortic interstitial cells (AVICs) exposed to cyclic stretch. Reducing HOTAIR levels via siRNA in AVICs results in increased expression of calcification genes.

2014-05-02 | E-MTAB-2409 | biostudies-arrayexpress

Changes in B10F10 melanoma cells in response to stretch

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Stretch-dependent genes in vascular smooth muscle cells

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Transcription profiling of rat organotypic hippocampal cultures following mild or severe stretch injury

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Changes in transcription profile in pelvic organ fibroblasts in response to stretch

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