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Metabolomics,Unknown,Transcriptomics,Genomics,Proteomics

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Expression data from pancreatic cancer cell line MiaPaca2

ABSTRACT: tumor-stroma crosstalk drives pancreatic carcinogenesis we used time-resolved genome-wide transcriptional profiling to analyse changes caused by co-exposure of pancreatic tumor and stellate cells pancreatic tumor cell line MiaPaca2 was treated with a supernatant of pancreatic stelalte cells, primed with cumulative TC-supernatant (of 8 tumor cell lines, TC) and harvested hourly at 1-7, and 24 hours post exposure for RNA extraction and hybridization on Affymetrix microarrays.

ORGANISM(S): Homo sapiens

SUBMITTER: Matt Rogon

PROVIDER: E-GEOD-49586 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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Expression data from pancreatic cancer cell line MiaPaca2 [control]

Project description:tumor-stroma crosstalk drives pancreatic carcinogenesis we used time-resolved genome-wide transcriptional profiling to analyse changes caused by co-exposure of pancreatic tumor and stellate cells pancreatic tumor cell line MiaPaca2 was treated with a supernatant of pancreatic stellate cells and harvested hourly at 1-7, and 24 hours post exposure for RNA extraction and hybridization on Affymetrix microarrays.

2013-08-09 | E-GEOD-49584 | biostudies-arrayexpress

Expression data from pancreatic stellate cells

Project description:tumor-stroma crosstalk drives pancreatic carcinogenesis we used time-resolved genome-wide transcriptional profiling to analyse changes caused by co-exposure of pancreatic tumor and stellate cells Primary pancreatic Stellate cells (PSC) were treated with a cumulative supernatant of pancreatic tumor cell lines (n=8) and harvested at 1-7, and 24 hours post exposure for RNA extraction and hybridization on Affymetrix microarrays. The 8 tumor cell lines are pancreatic ductal adenocarcinoma lines: AsPC1, BxPC3, Capan1, Colo357, MiaPaca2, Panc1, Su8686, and T3M4

2013-08-09 | E-GEOD-49583 | biostudies-arrayexpress

Expression data from pancreatic stellate cells and cancer cell line MiaPaca2

Project description:This SuperSeries is composed of the SubSeries listed below. Refer to individual Series

2013-08-09 | E-GEOD-49588 | biostudies-arrayexpress

Genes regulated by AGR2 in pancreatic cancer cell lines FA6 and MiaPaCa2

2013-07-28 | GSE12513 | GEO

Genes regulated by AGR2 in pancreatic cancer cell lines FA6 and MiaPaCa2

Project description:We and others have shown that AGR2 is frequently upregulated during the development of pancreatic cancer. We used microarray to look at the target genes regulated by AGR2 in pancreatic cancer cell lines FA6 and MiaPaCa2. Keywords: gene knock-down, overexpression We transiently down-regulated AGR2 expression in FA6 pancreatic cancer cells using INTERFERin transfection reagent and either AGR2 siRNA or non-targeting control siRNA for 48 hours. RNA was extracted and hybridized on Affymetrix microarrays. We looked for new target genes regulated by AGR2. We generated stable cell lines by introducing control vector pCEP4 or AGR2 overexpressing vector pCEP4-AGR2 into the pancreatic cancer cell line MiaPaCa2, single cell clones were then isolated. RNA was extracted and hybridized on Affymetrix microarrays.We looked for new target genes regulated by AGR2.

2013-07-28 | E-GEOD-12513 | biostudies-arrayexpress

Transcriptional profiling comparing MiaPaCa2 pancreatic cancer cells transfected with S100PBP siRNA to MiaPaCa2 cells transfected with non-targeting control siRNA

Project description:S100PBP is significantly down-regulated in pancreatic cancer compared to normal samples. The functional roles of S100PBP are unknown, and therefore, profiling cells after silencing of this gene may lead to further understanding of its functional mechanisms. We have performed microarray gene expression analysis of MiaPaCa2 cells treated with S100PBP siRNA compared to the same cells transfected with non-targeting control siRNA.

2014-01-01 | E-GEOD-35196 | biostudies-arrayexpress

Sensitivity of MiaPaCa2 cells to E2F inhibitor Ly101-4B.

Project description:MiaPaCa2 cells where screened with a set of original compounds with anticancer activity available at our laboratory. From these compounds we identified the nucleoside analogue ly101-4B as a strong inhibitor of the E2F activity. We confirmed its activity by studying its effect on MiaPaCa2's cell viability , caspase 3/7 activity and LDH release, to measure cell growth, apoptosis and necrosis after treatment with LY101-4B 25 µM. Then, to investigate the mechanism of action of ly101-4B we performed a transcriptomic analysis by using an Affymetrix approach (Genechip® Human Gene 1.0 ST Arrays).

2019-02-28 | GSE109853 | GEO

Expression data from pancreatic cancer cell line MiaPaca2

2013-08-09 | GSE49586 | GEO

Expression data from pancreatic cancer cell line MiaPaca2 [control]

2013-08-09 | GSE49584 | GEO

2',3,5-Trimethoxychalcone (MC-23)-induced gene expression in MiaPaCa2 cells

Project description:2',3,5-Trimethoxychalcone (MC-23) triggers apoptosis independently of p53 in MiaPaCa2 cells. To understand the mechanism of action of MC-23, we analysed the gene expression profiles in MiaPaCa2 cells treated with 10 μM MC-23 for 6, 12, or 24 h using the Agilent human 44K oligonucleotide V2 microarray. Out of the 34,127 genes in the array, MC-23 upregulated ~3% and downregulated. Notably, a large number of genes associated with apoptosis (such as BAX, BAK), chaperones (such as HSPA1/HSP70, HSPA5/GRP78/BiP), and the unfolded protein response (such as IRE1α, CHOP/DDIT3/GADD153, XBP1, ATF4, ATF6) were highly up-regulated by MC-23 treatment, suggesting that ER stress is associated with MC-23-induced apoptosis.

2014-01-21 | GSE54225 | GEO

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