Metabolomics,Unknown,Transcriptomics,Genomics,Proteomics

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Leucegene: AML sequencing

ABSTRACT: RNA sequencing of human leukemia The goals of this project are to obtain a comprehensive study of mutations and gene expression in human acute myeloid leukemia (AML). Methods: AML cells were thawed. DNA and RNA (polyA) was extracted and sequences were obtained with an Illumina HiSeq 2000 sequencer.

ORGANISM(S): Homo sapiens

SUBMITTER: Guy Sauvageau 

PROVIDER: E-GEOD-49642 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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Publications

RNA-Seq reveals spliceosome and proteasome genes as most consistent transcripts in human cancer cells.

Macrae Tara T   Sargeant Tobias T   Lemieux Sébastien S   Hébert Josée J   Deneault Eric E   Sauvageau Guy G  

PloS one 20130917 9

Accurate quantification of gene expression by qRT-PCR relies on normalization against a consistently expressed control gene. However, control genes in common use often vary greatly between samples, especially in cancer. The advent of Next Generation Sequencing technology offers the possibility to better select control genes with the least cell to cell variability in steady state transcript levels. Here we analyze the transcriptomes of 55 leukemia samples to identify the most consistent genes. Th  ...[more]

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