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Metabolomics,Unknown,Transcriptomics,Genomics,Proteomics

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Differential RNA-sequencing of Campylobacter jejuni fur perR mutant

ABSTRACT: We report the use of differential RNA-sequencing for the determination of the primary transcriptome of the fur perR mutant of Campylobacter jejuni NCTC 11168. This allows for the genome-wide determination of transcription start sites. Campylobacter jejuni NCTC 11168 fur perR mutant was grown to late log phase, RNA was purified and used for differential RNA-sequencing by 454 sequencing with barcoded libraries, and used for determination of genome-wide transcription start sites

ORGANISM(S): Campylobacter jejuni

SUBMITTER: Arnoud van Vliet

PROVIDER: E-GEOD-49660 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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RNA-sequencing of Campylobacter jejuni NCTC 11168 wildtype and fur perR mutant

Project description:We report the use of RNA-seq analysis for the determination of RPKM transcript levels in wildtype and fur perR mutant of Campylobacter jejuni NCTC 11168. This allows for comparison of gene expression levels. Campylobacter jejuni NCTC 11168 wildtype and fur perR mutant were grown to late log phase, RNA was purified and used for RNA-sequencing by Illumina HiSeq sequencing

2014-07-01 | E-GEOD-49687 | biostudies-arrayexpress

Differential RNA-sequencing of Campylobacter jejuni fur perR mutant

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2014-07-01 | GSE49660 | GEO

RNA-sequencing of Campylobacter jejuni NCTC 11168 wildtype and fur perR mutant

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Differential RNA-sequencing of Campylobacter jejuni NCTC 11168

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Project description:Comparison of the Campylobacter jejuni NCTC 11168 proteome in MH media in the presence and absence of Deoxycholate

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Wild Type 11168 versus LuxS01 Mutant Cultured in MHB and MEM- α media

Project description:Transcriptional profiling of Campylobacter jejuni NCTC 11168 wild type versus LuxS01 mutant strain grown in a minimal culture medium (MEM- α ) versus complex culture medium (MHB).

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Transcriptional profiles of Campylobacter jejuni NCTC 11168 to 4mg/L erythromycin treatment

Project description:Erythromycin is the drug of choice to treat campylobacteriosis, but resistance to this antibiotic is rising. The adaptive mechanisms employed by Campylobacter jejuni to erythromycin treatment remain unknown. The aim of this study is to determine the molecular basis underlying CampylobacterM-bM-^@M-^Ys immediate response to Ery treatment. The design utilized an available two color microarray slide for the entire transcriptome of Campylobacter jejuni wild type strain NCTC 11168. One hybridizations were performed: sham-treated NCTC 11168 v.s. lethal dose erythromycin treated NCTC 11168. Samples were independently grown and harvested. There were three biological replicates of each sample.

2013-10-10 | E-GEOD-43023 | biostudies-arrayexpress

Transcriptional profiles of Campylobacter jejuni NCTC 11168 to 0.125mg/L erythromycin treatment

Project description:Erythromycin is the drug of choice to treat campylobacteriosis, but resistance to this antibiotic is rising. The adaptive mechanisms employed by Campylobacter jejuni to erythromycin treatment remain unknown. The aim of this study is to determine the molecular basis underlying CampylobacterM-bM-^@M-^Ys immediate response to Ery treatment. The design utilized an available two color microarray slide for the entire transcriptome of Campylobacter jejuni wild type strain NCTC 11168. One hybridizations were performed: sham-treated NCTC 11168 v.s. sub-lethal dose erythromycin treated NCTC 11168. Samples were independently grown and harvested. There were three biological replicates of each sample.

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Alanine scan of 11 amino acid long novel linear epitope from C.jejuni cj0669 protein [2]

Project description:A short sequence of 11 amino acids belonging to the cj0669 protein from Campylobacter jejuni NCTC 11168 which was previously identified as potentially immunogenig was analyzed via alanine scanning to narrow down the significant amino acid residues within the sequence.

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