Identification of Lgr5-independent spheroid-generating progenitors of the mouse fetal intestinal epithelium

ABSTRACT: Immortal spheroids were generated from fetal mouse intestine using the culture system developed to culture organoids from adult intestinal epithelium. Spheroids are made of a monostratified polarized epithelium displaying a poorly differentiated intestinal phenotype. The proportion of spheroids generated from intestinal explants progressively decreases from fetal to postnatal period, with a corresponding increase in production of organoids. Spheroid cells show indefinite self-renewing properties but exhibit a transcriptome strikingly different from that of adult intestinal stem cells reminiscent of incompletely caudalized progenitors. The receptor Lgr4, but not Lgr5, is essential for their growth. Trop2/Tacstd2 and Cnx43/Gja1, two markers highly enriched in spheroids, are expressed through

ORGANISM(S): Mus musculus

SUBMITTER: Frederick Libert 

PROVIDER: E-GEOD-49803 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress