Dye Bias Array Set

ABSTRACT: Spot intensity serves as a proxy for gene expression in dual-label microarray experiments. Dye bias is defined as an intensity difference between samples labeled with different dyes attributable to the dyes instead of the gene expression in the samples. Dye bias that is not removed by array normalization can introduce bias into comparisons between samples of interest. But if the bias is consistent across the samples for the same gene, it can be corrected by proper experimental design and analysis. If the dye bias is not consistent across samples for the same gene, but is different for different samples, then removing the bias becomes more problematic, perhaps indicating a technical limitation to the ability of fluorescent signals to accurately represent gene expression. Thus, it is importa

ORGANISM(S): Homo sapiens

SUBMITTER: Kevin Dobbin 

PROVIDER: E-GEOD-4982 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress