Differential Protein Occupancy Profiling of the mRNA Transcriptome

ABSTRACT: Protein-RNA interactions are fundamental to core biological processes, such as mRNA splicing, localization, degradation and translation. We have developed a photoreactive nucleotide-enhanced UV crosslinking and oligo(dT) purification approach to identify the mRNA-bound proteome using quantitative proteomics and to display the protein occupancy on mRNA transcripts by next-generation sequencing (Baltz and Munschauer et al. 2012). Our current work focuses on streamlining and extending protein occupancy profiling on poly(A)-RNA. Our objectives are to identify previously unknown protein-bound transcripts and, more importantly, to assess global and local differences in protein occupancy across different biological conditions. To this end, we have implemented poppi, the first pipeline for differe

ORGANISM(S): Homo sapiens

SUBMITTER: Markus Landthaler 

PROVIDER: E-GEOD-49831 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress