Metabolomics,Unknown,Transcriptomics,Genomics,Proteomics

Dataset Information

A Parallel Study of mRNA and MicroRNA Profiling of Peripheral Blood in Young Adult Women (mRNA Profiling)

ABSTRACT: The goal of this study is to identify aging-related biomarkers to improve our understanding of complex physiological changes, thereby providing a means to investigate the mechanism by which aging influences various diseases. Global gene expression patterns from peripheral blood of two groups of healthy young adult women, among which 13 were aged 22-25 and 9 were aged 36-39 years old, were compared to determine changes in gene expression that are associated with aging.

ORGANISM(S): Homo sapiens

SUBMITTER: Samantha Gadd

PROVIDER: E-GEOD-49839 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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Publications

A Parallel Study of mRNA and microRNA Profiling of Peripheral Blood in Young Adult Women.

Sredni Simone T ST Gadd Samantha S Jafari Nadereh N Huang Chiang-Ching CC

Frontiers in genetics 20110718

<h4>Background</h4>Aging is a complex process that involves the interplay of genetic, epigenetic, and environmental factors. Identifying aging-related biomarkers holds great potential for improving our understanding of complex physiological changes, thereby providing a means to investigate the mechanism by which aging influences various diseases.<h4>Method and results</h4>We performed a parallel study of microRNA and gene expression profiling of peripheral blood in a group of healthy young adult ...[more]

PMID: 22303345

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Project description:Room temperature whole blood mRNA stabilization procedures, such as the PAX gene system, are critical for the application of transcriptional analysis to population-based clinical studies. Global transcriptome analysis of whole blood RNA using microarrays has proven to be challenging due to the high abundance of globin transcripts that constitute 70% of whole blood mRNA in the blood. This is a particular problem in patients with sickle-cell disease, secondary to the high abundance of globin-expressing nucleated red blood cells and reticulocytes in the circulation . In order to more accurately measure the steady state whole blood transcriptome in sickle-cell patients, we evaluated the efficacy of reducing globin transcripts in PAXgene stabilized RNA samples for genome-wide transcriptome analyses using oligonucleotide arrays. We demonstrate here by both microarrays and Q-PCR that the globin mRNA depletion method resulted in 55-65 fold reduction in globin transcripts in whole blood collected from healthy volunteers and sickle-cell disease patients. This led to an improvement in microarray data quality with increased detection rate of expressed genes and improved overlap with the expression signatures of isolated peripheral blood mononuclear (PBMC) preparations. The differentially modulated genes from the globin depleted samples had a higher correlation coefficient to the 112 genes identified to be significantly altered in our previous study on sickle-cell disease using PBMC preparations. Additionally, the analysis of differences between the whole blood transcriptome and PBMC transcriptome reveals important erythrocyte genes that participate in sickle-cell pathogenesis and compensation. The combination of globin mRNA reduction after whole-blood RNA stabilization represents a robust clinical research methodology for the discovery of biomarkers for hematologic diseases and in multicenter clinical trials investigating a wide range of nonhematologic disorders where fractionation of cell types is impracticable. Keywords: Microarrays, PAXgene, globin reduction, whole blood, PBMC There are 10 samples for each of PBMC, PAX and PAX globin-reduced, where 5 samples come from sickle-cell patients and 5 from healthy controls.

Dataset Information

A Parallel Study of mRNA and MicroRNA Profiling of Peripheral Blood in Young Adult Women (mRNA Profiling)

Publications

A Parallel Study of mRNA and microRNA Profiling of Peripheral Blood in Young Adult Women.

Similar Datasets

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