RNA-Seq and expression microarray highlight different aspects of the fetal amniotic fluid transcriptome [microarray]

ABSTRACT: The second trimester fetal transcriptome can be assessed based on cell-free RNA found within the amniotic fluid supernatant. The objective of this study was to compare the suitability of two technologies for profiling the human fetal transcriptome: RNA-Seq and expression microarray. Comparisons were based on total numbers of gene detected, rank-order gene expression, and functional genomic analysis. Fewer gene transcripts were observed using RNA-Seq than microarray (4,158 vs 8,842). Correlation of total expression within each sample ranged from R=0.43 to R=0.57. On average, there was 59% concordance in gene identity among the top 10% of genes ranked by expression. The RNA-Seq data yielded more significant pathways enrichment within the ?Physiological Systems Development and Function? categ

ORGANISM(S): Homo sapiens

SUBMITTER: Lillian Zwemer 

PROVIDER: E-GEOD-49891 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress