DNA intermediates of meiotic recombination in synchronous S. pombe at optimal temperature

ABSTRACT: Crossovers formed by recombination between homologous chromosomes are important for proper homolog segregation during meiosis and for generation of genetic diversity. Optimal molecular analysis of DNA intermediates of recombination requires synchronous cultures. We previously described a mutant, pat1-as2, of the fission yeast Schizosaccharomyces pombe that undergoes synchronous meiosis at 25°C when an ATP analog is added to the culture. Here, we compare recombination intermediates in pat1-as2 at 25°C with those in the widely used pat1-114 temperature-sensitive mutant at 34°C, a temperature higher than optimal. DNA double-strand breaks at most hotspots are similarly abundant in the two conditions but, remarkably, a few hotspots are distinctly deficient at 25°C. In both conditions H

ORGANISM(S): Schizosaccharomyces pombe

SUBMITTER: Kyle Fowler 

PROVIDER: E-GEOD-49961 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress