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Metabolomics,Unknown,Transcriptomics,Genomics,Proteomics

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Gene expression of the mouse choroid plexus epithelium (CPE)

ABSTRACT: We investigated the gene expression of the mouse CPE. We isolated CPE cells from three mouse brains with laser dissection microscopy. Next, we performed RNA isolation, amplification, labeling and hybridization against 44k Agilent microarrays. We performed the microarrays against a common reference sample, which was mouse RPE/choroid RNA. We performed 3 mouse CPE replicates from 3 different mouse from the same strain, namely C57BL/6 .

ORGANISM(S): Mus musculus

SUBMITTER: Sarah Janssen

PROVIDER: E-GEOD-49973 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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Gene expression of the human choroid plexus epithelium (CPE)

Project description:We investigated the gene expression of the human CPE.We isolated CPE cells from healthy human donor choroid plexus tissues with laser dissection microscopy. Next, we performed RNA isolation, amplification, labeling and hybridization against 44k Agilent microarrays. We performed the microarrays against a common reference sample, namely human RPE/choroid RNA. We performed 7 replicates of human CPE samples from 7 different donors.

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RNA-Seq of native and FusionRed-transfected canine tumor cell lines after 3 hours C-CPE treatment

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Gene expression of the pigmented and non-pigmented epithelia of the ciliary body of human healthy eyes

Project description:The ciliary body (CB) of the human eye consists of the non-pigmented (NPE) and pigmented (PE) neuro-epithelia. We investigated the gene expression of the NPE and PE, to shed light on the molecular mechanisms underlying the most important functions of the CB. Therefore we isolated NPE and PE cells from seven healthy human donor eyes using laser dissection microscopy. Next, we performed RNA isolation, amplification, labeling and hybridization against 44k Agilent microarrays. We performed the microarrays against a common reference sample, namely human RPE/choroid RNA. We performed 7 replicates of PE samples from 7 different donors and 7 NPE replicates from the same 7 different donors.

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