Distinct properties of cell type-specific and shared transcription factor binding sites

ABSTRACT: Most human transcription factors bind a small subset of potential genomic sites and often use different subsets in different cell types. To identify mechanisms that govern cell type-specific transcription factor binding, we used an integrative approach to study estrogen receptor α (ER). We found that ER exhibits two distinct modes of binding. Shared sites, bound in multiple cell types, are characterized by high affinity estrogen response elements (EREs), inaccessible chromatin and a lack of DNA methylation, while cell-specific sites are characterized by a lack of EREs, co-occurrence with other transcription factors and cell type-specific chromatin accessibility and DNA methylation. These observations enabled accurate quantitative models of ER binding that suggest tethering of ER to one

ORGANISM(S): Mus musculus

SUBMITTER: Jason Gertz 

PROVIDER: E-GEOD-49993 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress