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Metabolomics,Unknown,Transcriptomics,Genomics,Proteomics

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MRNA and RNC-mRNA deep sequencing of three hepatocellular carcinoma cell lines

ABSTRACT: We sequenced the total mRNA and translating mRNA (RNC-mRNA) of three hepatocellular carcinoma cell lines Hep3B, HCCLM3 and MHCC97H For each cell line, samples prepared from three independent and identical cell cultures were pooled with equal amounts. C-HPP China Team

ORGANISM(S): Homo sapiens

SUBMITTER: Gong Zhang

PROVIDER: E-GEOD-49994 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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Systematic analysis of missing proteins provides clues to help define all of the protein-coding genes on human chromosome 1.

Zhang Chengpu C Li Ning N Zhai Linhui L Xu Shaohang S Liu Xiaohui X Cui Yizhi Y Ma Jie J Han Mingfei M Jiang Jing J Yang Chunyuan C Fan Fengxu F Li Liwei L Qin Peibin P Yu Qing Q Chang Cheng C Su Na N Zheng Junjie J Zhang Tao T Wen Bo B Zhou Ruo R Lin Liang L Lin Zhilong Z Zhou Baojin B Zhang Yang Y Yan Guoquan G Liu Yinkun Y Yang Pengyuan P Guo Kun K Gu Wei W Chen Yang Y Zhang Gong G He Qing-Yu QY Wu Songfeng S Wang Tong T Shen Huali H Wang Quanhui Q Zhu Yunping Y He Fuchu F Xu Ping P

Journal of proteome research 20131125 1

Our first proteomic exploration of human chromosome 1 began in 2012 (CCPD 1.0), and the genome-wide characterization of the human proteome through public resources revealed that 32-39% of proteins on chromosome 1 remain unidentified. To characterize all of the missing proteins, we applied an OMICS-integrated analysis of three human liver cell lines (Hep3B, MHCC97H, and HCCLM3) using mRNA and ribosome nascent-chain complex-bound mRNA deep sequencing and proteome profiling, contributing mass spect ...[more]

PMID: 24256544

Publication: 1/4

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