Metabolomics,Unknown,Transcriptomics,Genomics,Proteomics

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ABSTRACT: Protein microarrays for highly parallel detection and quantitation of specific proteins and antibodies in complex solutions. This study is described more fully in Haab, Dunham, and Brown, Genome Biology 2001 2(2): research0004.1-0004.13. Keywords: other

ORGANISM(S): Homo sapiens

SUBMITTER: B Haab 

PROVIDER: E-GEOD-50 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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Protein microarrays for highly parallel detection and quantitation of specific proteins and antibodies in complex solutions.

Haab B B BB   Dunham M J MJ   Brown P O PO  

Genome biology 20010122 2


<h4>Background</h4>We have developed and tested a method for printing protein microarrays and using these microarrays in a comparative fluorescence assay to measure the abundance of many specific proteins in complex solutions. A robotic device was used to print hundreds of specific antibody or antigen solutions in an array on the surface of derivatized microscope slides. Two complex protein samples, one serving as a standard for comparative quantitation, the other representing an experimental sa  ...[more]

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