Applied Biosystems Human TaqMan Low Density Array (TLDA) in plasma samples from HCC cases and controls

ABSTRACT: MicroRNAs (miRNAs) are abundant in the circulation and play a central role in diverse biological processes; they may be useful for early diagnosis of hepatocellular carcinoma (HCC). We conducted a two-phase, case-control study (20 pairs for the discovery set and 49 pairs for the validation set) to test the hypothesis that genome-wide dysregulation of circulating miRNAs differentiate HCC cases from controls. Taqman low density arrays were used to examine genome-wide miRNA expression for the discovery set, and quantitative RT-PCR was used to validate candidate miRNAs for both discovery and validation sets. Sixty-six miRNAs were found to be significantly over-expressed in plasma of HCC cases compared to controls after adjusting for false discovery rate (p<0.05). A volcano plot indicated that

ORGANISM(S): Homo sapiens

SUBMITTER: Jing Shen 

PROVIDER: E-GEOD-50013 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress