Six1 homeoprotein drives myofiber type IIA specialization in soleus muscle

ABSTRACT: Six homeoproteins regulate fast MYH expression and calcium homeostasis soleus muscles were collected from cSix1 KO and control mice. Total RNAs were extracted by Trizol Reagent (Invitrogen) according to manufacturer's instruction. After validation of RNA quality with the Bioanalyzer 2100 (using Agilent RNA6000 nano chip kit), 50 ng of total RNA were reverse transcribed following the Ovation PicoSL WTA System (Nugen). Briefly, the resulting double-strand cDNA was used for amplification based on SPIA technology. After purification according to Nugen protocol, 5 μg of single strand DNA was used for generation of Sens Target DNA using Ovation Exon Module kit (Nugen). 2.5 μg of Sens Target DNA were fragmented and labelled with biotin using Encore Biotin Module kit (Nugen). After control of fr

ORGANISM(S): Mus musculus

SUBMITTER: iori sakakibara 

PROVIDER: E-GEOD-50023 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress