Long-range chromatin contacts reveal a role for the pluripotency and Polycomb networks in genome organization

ABSTRACT: We mapped long-range chromatin interactions in embryonic stem cells (ESCs), iPSCs, and fibroblasts, and uncovered an ESC-specific genome organization that is gradually re-established during reprogramming. Confirming previous results, we show that open, accessible and closed chromatin character is the primary determinant of long-range chromatin interaction preferences. Importantly, we find that in ESCs, genomic regions extensively occupied by the pluripotency factors Oct4, Sox2, and Nanog preferentially co-localize. Similarly, regions strongly enriched for Polycomb-proteins and H3K27me3 frequently interact, and loss of the Polycomb-protein Eed diminishes these interactions without dramatically changing overall chromosome-conformation. Consistent with a spatial segregation of transcriptional

ORGANISM(S): Mus musculus

SUBMITTER: Kathrin Plath 

PROVIDER: E-GEOD-50029 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress