Histone H3 Ser10 phosphorylation in hpr1-101

ABSTRACT: R-loops are transcription by-products that may constitute a threat to genome integrity.We previously show in Saccharomyces cerevisiae that R-loops are tightly and specifically linked with histone H3-Ser10 phosphorylation (H3S10P), a mark of chromatin condensation. Here we analyse the hpr1-101 mutant. A point mutation that impairs transcription and mRNP biogenesis without increasing recombination. Importantly, ChIP-chip analyses reveal a clear H3S10P does not accumulate at the pericentromeric chromatin during the G1-phase of the cell cycle but during S-phase in non-accumulating R-loop hpr1-101. ChIP-chip studies were perfomed with antibodies against Histone H3 and the phosphorylated Histone H3 at Serine10 in the yeast S. cerevisiae.

ORGANISM(S): Saccharomyces cerevisiae

SUBMITTER: Andrés Aguilera 

PROVIDER: E-GEOD-50110 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress