Altering gene expression by aminocoumarin (novobiocin) treatment: Role of DNA supercoiling in Staphylococcus aureus

ABSTRACT: For Staphylococcus aureus it was shown previously that aminocoumarinecoumarin antibiotics such as novobiocin lead to immediate down-regulation of recA expression and thereby inhibition of the SOS response, the mutation frequency and the recombination capacity. Aminocoumarinecoumarin function by inhibition of the ATPase activity of the gyrase subunit B. Here we analysed the global impact of the DNA relaxing agent novobiocin on gene expression in S. aureus. By use of a novobiocin resistant mutant, it became evident that the change in recA expression is due to gyrase inhibition. Microarray analysis and Northern blot hybridization revealed that the expression of a distinct set of genes is increased (e.g. recF-gyrB-gyrA, rib operon and ure operon M-bM-^@M-&)), or decreased (e.g. arlRS, recA, l

ORGANISM(S): Staphylococcus aureus

SUBMITTER: Ludger Klein-Hitpass 

PROVIDER: E-GEOD-50157 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress