Metabolomics,Unknown,Transcriptomics,Genomics,Proteomics

Dataset Information

Expression data from rat cardiac fibroblasts

ABSTRACT: The overall data contains expression profile of rat genes of cardiac fibroblasts. G-protein signaling is regulated in cardiac fibroblasts. The expresssion of angiotensin II receptor or cytokine such as interleukin is regulated by PTX (which is suggested to suppress Gi function) treatment. Keywords: control or PTX treatment Rat cardiac fibroblasts were primarily isolated and plated onto 6 well plate. Cells were treated with water (Ctl 1-3) or PTX for 24 hr (PTX 1-3) and lysed by Buffer RLT. Total RNA was isolated using QIAGEN kit and gene expression was analysed using Affymetrix Gene Chip system and GCOS analysis.

ORGANISM(S): Rattus norvegicus

SUBMITTER: Hitoshi Kurose

PROVIDER: E-GEOD-5017 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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Publications

Pertussis toxin up-regulates angiotensin type 1 receptors through Toll-like receptor 4-mediated Rac activation.

Nishida Motohiro M Suda Reiko R Nagamatsu Yuichi Y Tanabe Shihori S Onohara Naoya N Nakaya Michio M Kanaho Yasunori Y Shibata Takahiro T Uchida Koji K Sumimoto Hideki H Sato Yoji Y Kurose Hitoshi H

The Journal of biological chemistry 20100315 20

Pertussis toxin (PTX) is recognized as a specific tool that uncouples receptors from G(i) and G(o) through ADP-ribosylation. During the study analyzing the effects of PTX on Ang II type 1 receptor (AT1R) function in cardiac fibroblasts, we found that PTX increases the number of AT1Rs and enhances AT1R-mediated response. Microarray analysis revealed that PTX increases the induction of interleukin (IL)-1beta among cytokines. Inhibition of IL-1beta suppressed the enhancement of AT1R-mediated respon ...[more]

PMID: 20231290

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Dataset Information

Expression data from rat cardiac fibroblasts

Publications

Pertussis toxin up-regulates angiotensin type 1 receptors through Toll-like receptor 4-mediated Rac activation.

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