Transcription profiling of zebrafish embryonic retinal pigment epithelium in vivo

ABSTRACT: Eye development and photoreceptor maintenance requires the retinal pigment epithelium (RPE), a thin layer of cells that underlies the neural retina. Despite its importance, RPE development has not been studied by a genomic approach. A microarray expression profiling methodology was established in this study for studying RPE development. The intact retina with RPE attached was dissected from developing embryos, and differentially expressed genes in RPE were inferred by comparing the dissected tissues with retinas without RPE using microarray and statistical analyses. We found 8810 probesets to be significantly expressed in RPE at 52 hours post-fertilization (hpf), of which 1443 might have biologically meaningful expression levels. Further, 78 and 988 probesets were found to be significantly

ORGANISM(S): Danio rerio

SUBMITTER: Yuk Fai Leung 

PROVIDER: E-GEOD-5048 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress